A total of 5 × 106 transfected SW480 cells were subcutaneously injected into six-week-old male nude mice (n = 5 per group). Tumor volume was measured every 3 days according to the following formula: volume = 1/2 × length × width2. Animal protocols, housing, and care were performed with the approval of the Ethics Committee of Xi’an Central Hospital and conducted according to the guidelines set forth in the National Institutes of Health’s (NIH) “Guide for the Care and Use of Laboratory Animals” (8th edition).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.