Cell morphology quantification

For each transfection, a representative phase-contrast image at a single time point was exported from the Incucyte time-series. These raw images were first transformed to probability maps of cells by pixel classification using ilastik (1.3.2) (Berg et al. 2019). The trained model was then applied to all images where the predicted probability maps of cells (grayscale, 16 bits tiff format) were subsequently used for morphology quantification in CellProfiler (3.1.5) (Carpenter et al. 2006). For details, please refer to the Supplemental Methods.