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2.4. In Vivo Bioluminescence Imaging

XS Xiao-Hai Song
XC Xin-Zu Chen
XC Xiao-Long Chen
KL Kai Liu
WZ Wei-Han Zhang
XM Xian-Ming Mo
JH Jian-Kun Hu
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Mice were sedated with 4% chloral hydrate (400 mg/kg) and given a single intraperitoneal (i.p.) 150 mg/kg dose of D-luciferin (Nanjing Asian Chemical Co., Ltd., China) in PBS. Bioluminescence imaging with a CCD camera (Xenogen, U.S.) was initiated 10 minutes after injection of D-luciferin. The imaging time ranged from 1 to 60 seconds according to the amount of luciferase activity.

Bioluminescence was assessed in the region of interest (ROI), which was defined manually. All data are expressed in units of photon flux (photons/s/cm2/steradian) and were collected and analyzed using the in vivo imaging system (Xenogen, U.S.).

Copyright and License information:  ©2020 Xiao-Hai Song et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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