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MW, pI and hydrophobicity calculation
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Molecular weight, pI, and hydrophobicity were computed by custom PERL scripts. For molecular weight, monoisotopic masses were used (as, e.g., listed in Expasy (https://web.expasy.org/findmod/findmod_masses.html#AA)). Computation of pI values is based on the pK values for amino acids at internal, N-terminal, and C-terminal positions105. For hydrophobicity, the GRAVY index was computed, based on the hydropathy index of amino acids106.
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