SRBSDV detection by RT-PCR

Virus infection status was detected by one-step RT-PCR [16]. Briefly, total RNA of each sample was extracted using the methods provided by the manufacturer of RNAiso Plus (Takara Biotechnology Co., Ltd, Dalian, China) [16]. The extracted RNA were amplified using primers (forward: 5’-cgcgtcatct caaactacag-3’, reverse: 5’- tttgtcagcatctaaagcgc-3’) [17] and PrimeScript One Step RNA RT-PCR Kit Ver.2 (Takara Biotechnology Co., Ltd, Dalian, China) according to the manufacturer’s instructions. The amplified fragment of the expected size (682 bp) of SRBSDV-S10 fragment (GenBank acc. No.{"type":"entrez-nucleotide","attrs":{"text":"EU523360.1","term_id":"183229402","term_text":"EU523360.1"}}EU523360.1) was confirmed by electrophoresis in 1% (w/v) agarose gels.