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2.2. pDC isolation and culture

YY Yishan Ye
LR Laure Ricard
LS Lama Siblany
NS Nicolas Stocker
FV Frédéric De Vassoigne
EB Eolia Brissot
BL Baptiste Lamarthée
AM Arsène Mekinian
MM Mohamad Mohty
BG Béatrice Gaugler
FM Florent Malard
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Peripheral blood mononuclear cells (PBMCs) were obtained from buffy coats by Ficoll density centrifugation, from healthy donors (Etablissement Français du Sang, Paris Saint-Antoine-Crozatier, France) or untreated SSc patients (Hôpital Saint-Antoine, Paris, France) after informed consent. The study was approved by the local institutional review board and the Comité de Protection des Personnes Ile-de France VII (CPP Ouest-1, reference 2017-A03380-53). pDCs were negatively selected with EasySep™ Human Plasmacytoid DC Enrichment Kit (Stem cell, Grenoble, France). The purity of isolated pDCs, verified by flow cytometry using PE-Vio770-BDCA2 (Miltenyi Biotec), ECD-CD123 (Beckman Coulter, Villepinte, France), was >90%.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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