3.5. Sephadex LH-20 Fractionation

The SL-A extract (3.0282 g) was fractionated onto Sephadex LH-20 column (60 x 3 cm), eluted first with water (800 mL) and after with 20% (250 mL), 40% (100 mL), 80% (150 mL), and 100% MeOH (2000 mL) in water; next, the eluents were 25% (150 mL), 50% (150 mL), and 100% acetone (150 mL) in MeOH. Column eluates were pooled according to TLC analysis in nine fractions: A-1 (0.1101 g), A-2 (0.0876 g), A-3 (0.1821 g), A-4 (0.1326 g), A-5 (0.5968g), A-6 (0.4303 g), A-7 (0.8807 g), A-8 (0.1582 g), and A-9 (0.2503 g), with a total weight of 2.8287 g (93% of total extract recovered).