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We synchronized the 82-6-hTert and A549 cells at the G1/S transition using a single thymidine treatment. Cells were incubated in the presence of thymidine (2 mM) for 18 h. After washing using pre-warmed PBS twice, fresh growth medium was supplied to allow cells to progress in the cell cycle. At 7 h after release, G2-enriched cells were irradiated and collected for western blotting, either immediately or at the indicated times thereafter.
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