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To measure the activity of HDACs we utilized the Epigentek ‘EpiQuik HDAC Activity / Inhibition Assay Kit’ (Epigentek, via Biocat). The procedure was performed as recommended in the manual. Whole protein lysates were prepared by NP-40 isolation buffer (150 mM sodium chloride, 1% NP-40, 50 mM Tris (pH 8)). 10 μg of protein was used for analysis.
Copyright and License information: ©2016 Nettersheim et al.
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