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OVCAR3 and CAOV-3 cells were added to a 96-well dish at 1×103 cells/well and were then transfected with 50 nM miR-196 mimic, miR-NC mimic, miR-196 inhibitor, miR-NC inhibitor, pcDNA-DDX3, pcDNA-3.1, shDDX3 or shCtrl. Subsequently, 10 µl MTT solution was added to each well at 0, 24, 48, 72 and 96 h. The supernatant was discarded after 4 h of incubation at 37°C and then 100 µl DMSO was added to each well to dissolve the formazan. The absorbance at 570 nm was determined using an enzyme-labeling instrument (EL808; BioTek Instruments, Inc.).
Copyright and License information: ©2020 Ni et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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