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Shp2 siRNA preparation and transfection
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Shp2-specific siRNA interference was performed as previously reported [11]. Sequences of the oligonucleotides are as follows: 5′-GAACAUCACGGCAAUUAAUU-3′; 5′-GAACACUGGUGAUUACUAUUU-3′. The cells were cultured in 24-well plates for 24 h. The Shp2-specific or control siRNA was transfected into NCI-H292 cells using Lipofectamine LTX (Invitrogen) according to the manufacturer’s instructions. Immunoblotting analysis was used to validate Shp2 silencing by siRNA at 72 h after transfection.
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