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2.3. Immunophenotypic Analyses
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Immunophenotypic analyses were performed using bone marrow samples at diagnosis by flow cytometry. Cells were stained with anti-CD45 monoclonal antibody (mAb), gated by CD45 expression and side scatter (SSC), and analyzed by fluorescein conjugated mAb against CD2, CD5, CD7, CD4, CD8, CD19, CD20, CD11b, CD13, CD14, CD15, CD33, CD34, CD56 and HLA-DR antigens. In accordance with the EGIL criteria [28], surface markers generally not determined on APL cells were defined as positive if more than 10% of APL cells expressed the corresponding antigens.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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