siRNA‐control (siCon), and ANGPTL4 siRNA 1 and 2 (siANGPTL4) were purchased from Dharmacon (On‐TARGET plus‐mouse SMART‐pool ANGPTL4, L‐56622‐00‐0010) and Invitrogen (Stealth mouse ANGPTL4 siRNA MSS226640), respectively. Cells were transfected with siRNA using Lipofectamine RNAiMAX transfection reagent (Invitrogen) according to the manufacturer's protocols.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.