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HIF-1α inhibition by YC-1 in CoCl2-induced hypoxia
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To validate the effect of YC-1, a HIF-1α inhibitor (Santa Cruz Biotechnology, Santa Cruz, CA, USA), cells were cultured with 100 μM YC-1 for 24 h in CoCl2-induced hypoxic conditions and HIF-1α nuclear localization was assessed. Next, to investigate whether HIF-1α regulated collagen type I (COL1) gene expression in our system, we treated cells cultured in CoCl2-induced hypoxic conditions (12-well plates, 3 × 105 cells/cm2) with 100 μM YC-1 for 24 h. COL1 gene expression was assessed by RT-PCR.
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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
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