2.6. Chromatin immunoprecipitation‐quantitative PCR (ChIP‐qPCR)

Experiments were performed in a 10‐cm dish scale using the Pierce Agarose ChIP Kit (Thermo Scientific, 26156) according to the manufacturer's instructions with minor modifications. Briefly, agarose beads were used to pre‐binding overnight with antibodies against TET1 (Millipore Sigma, ABE1034), H3K4me3 (Cell signaling, C42D8), and H3K27me3 (Cell signaling, C36B11). IgG was included as a negative control. Cells were cross‐linked with 1% of formaldehyde at RT for 10 minutes, and the reaction was stopped by 1× glycine. ChIPs were carried out overnight at 4°C. Primers (Table S1) for the promoter regions of TGFBR2 and TSP1 were used to amplify input and ChIP‐purified DNA. The relative enrichments of the indicated DNA regions were calculated using the Percent Input Method according to the manufacturer's instructions and were normalized to % input.