2.9. In vivo cytotoxicity assay

TA Takahiro Aoki
MT Mariko Takami
TT Tomozumi Takatani
KM Kiwamu Motoyoshi
AI Ayana Ishii
AH Ayaka Hara
TT Takahide Toyoda
RO Reona Okada
MH Moeko Hino
RK Ryo Koyama‐Nasu
MK Masahiro Kiuchi
KH Kiyoshi Hirahara
MK Motoko Y. Kimura
TN Toshinori Nakayama
NS Naoki Shimojo
SM Shinichiro Motohashi
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Six‐week‐old to eight‐week‐old female NOG mice were intravenously inoculated with 1 × 106 K562 cells resuspended in 200 μL PBS per mouse. On the following day, each mouse was intravenously injected with 4 × 106 purified iNKT cells (in the treatment group) or PBS (in the control group). Bodyweight was monitored twice a week. Mice were humanely euthanized if they lost more than 20% of their peak weight.

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