Patients' blood samples were taken preoperatively each with 3 serum pipes. IL-6, CRP, and leukocytes were measured by the intern laboratory medicine. The other samples were frozen and collected for leptin enzyme-linked immunosorbent assay (ELISA) and oxidative stress level determination. Serum leptin levels were determined using a commercially available Human Leptin Quantikine ELISA Kit (R&D Systems), following the instructions of the manufacturer.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.