DNA extraction and Plasmodium vivax confirmation

SM Sayed Hussain Mosawi
AD Abdolhossein Dalimi
NS Najibullah Safi
RF Reza Fotouhi-Ardakani
FG Fatemeh Ghaffarifar
JS Javid Sadraei
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Disks (2 mm in diameter) were punched out from each DBCs and washed 3 times with DBCs purification buffer and twice with distilled water. The disks were air dried and used directly as template of PCR processing [29]. A semi-nested multiplex PCR were performed on each sample for final confirmation of P. vivax [30].

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