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NFAT gene expression

YY Yi-Chun Yeh
YL Yu-Ping Lin
HK Holger Kramer
AP Anant B Parekh
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HEK239T cells were co-transfected with either WT Orai1, Orai1–SNPs or the double variant together with an NFAT promoter-driven EGFP-based reporter plasmid. 24 hours after transfection, cells were stimulated with 500 nM thapsigargin in medium for 4 minutes and then thapsigargin was washed and cells incubated with medium for another 24 hours. The percentage of cells that were positive for GFP was then measured.

Copyright and License information:  ©2019 The Author(s) 2020. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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