Synergy Analysis of Drug Combination

CompuSyn software (version 2.0; ComboSyn, Inc., Paramus, NJ) was used to evaluate the synergistic effects of the combination of FKA and doxorubicin using the median-effect method [22]. MTT assays were performed to determine the fraction of cells affected (Fa). The combination index (CI) and dose-reduction index (DRI) were calculated using CompuSyn. In this analysis, the combined effect was reported as synergistic, antagonistic, or additive when the Log(CI) value was <0, >0, and 0, respectively. The drug dose-reduce inclination was reported as favorable dose reduction, not favorable dose reduction, or no dose reduction when the Log(DRI) value was >0, <0, and 0, respectively.