2.7. Cell Cycle and Cell Death Analysis by Flow Cytometry

LNCaP and PC-3 cells were seeded in 6-well plates and treated 48 h after seeding, then harvested 6 days after. For cell cycle analysis, live cells were fixed for 24 h in 70% ethanol and incubated for 30 min at RT with 100 μg/mL RNAse A and 25 μg/mL propidium iodide. For cell death analysis, cells were incubated 5 min at RT with DRAQ7 (ab109202, Abcam Inc., Cambridge, MA, USA). A maximum of 30,000 events were counted per condition using the Fortessa flow cytometer (BD Biosciences, Mississauga, ON, Canada) and analyzed with FlowJo software (Ashland, OR, USA, V10).