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Specimens and preparation for scanning electron microscope (SEM)
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A 5-mm segment was cut-off from the end of the SV before distension and then fixed by 2% paraformaldehyde. After distention with heparinized papaverine saline, another 5-mm segment was taken from the end of the SV for fixing. Those specimens were post-fixed in 1% osmium tetroxide in phosphate buffer. Then the specimens were spatter-coated with a gold layer and observed with a JEOL JSM5510 SEM.
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