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Preparation of sodium pervanadate
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To create a 50 mM pervanadate stock solution, 30% H2O2 was first diluted to 0.3% H2O2 in 20 mM HEPES, pH 7.3. Fifty microliters of dilute H2O2 added to 490 μl 100 mM sodium orthovanadate (Alfa Aesar) and 450 μl H2O, then mixed by gentle inversion and incubated at RT for 5 min. After incubation, excess H2O2 was quenched by the addition of a small amount of catalase (using 200 μl pipette tip) and mixed by gentle inversion. Pervanadate was freshly prepared and used immediately to avoid decomposition.
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