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Animals, experimental procedure, and treatments

HM Hongxia Mei
YT Ying Tao
TZ Tianhao Zhang
FQ Feng Qi
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Experiments were performed on adult male Sprague Dawley rats (250–300 g; Shanghai Experimental Animal Center of China). Rats were provided with water and food ad libitum. The use of animals in this study was approved by Animal Studies Ethics Committee of the Second Affiliated Hospital of Wenzhou Medical University.

Rats were randomized into five groups (n = 6): control group, LPS group, LPS + Emo 5 mg/kg group, LPS + Emo10 mg/kg group and LPS + Emo 20 mg/kg group. In LPS group, rats received LPS (20 mg/kg) through the tail vein. LPS was dissolved in 0.9% normal saline. In the Emo group, rats received Emo (5 mg/kg, 10 mg/kg and 20 mg/kg) via intraperitoneal injection 30 min before LPS exposure. Emo was dissolved in 100% DMSO at a concentration of 200 mg/mL and diluted in saline to the final concentration of 1 mg/ml. The action time of LPS-induced acute lung injury in rats was 4 h.

Copyright and License information: The Author(s) ©2020
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