NanoString nCounter mRNA Profiling

NanoString gene-expression analysis was performed using the profile using the NanoString nCounter Human Immunology Panel (NanoString Technologies, Seattle, WA, USA). Total RNA was extracted from normal human dendritic cells cultured in the presence or absence of arginase-rich supernatant from 293T-transfected cells. RNA extraction was performed by the Wistar Genomics Core. RNA samples were profiled according to standard NanoString protocols on a NanoString nCounter Analysis System operated by the Wistar Genomics Core. Data were analyzed using NanoString nSolver software.