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Assembly and evaluation of IC RDT

KS Keita Suzuki
RH Ralph Huits
JP Juthamas Phadungsombat
AT Aekkachai Tuekprakhon
EN Emi E. Nakayama
RB Riemsdijk van den Berg
BB Barbara Barbé
LC Lieselotte Cnops
RR Rummana Rahim
AH Abu Hasan
HI Hisahiko Iwamoto
PL Pornsawan Leaungwutiwong
ME Marjan van Esbroeck
MR Mizanur Rahman
TS Tatsuo Shioda
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The details of components of the 1st version (version A) have been described previously [25]. Briefly, the rapid IC RDT incorporated two mouse anti-CHIKV MAbs: CK47 was immobilized onto the membrane at the test line and used for CHIKV antigen-capture; CK119 was conjugated to AuNPs and placed at the conjugated pad by TANAKA Kikinzoku Kogyo K. K, Japan. The 2nd (versions B, C, D, E, F, M, N) and 3rd (version O) -generation IC RDTs were assembled with the combinations of MAbs shown in Table 1.

List of antibody combinations

Thirty microliters of serially diluted culture supernatant containing CHIKV or CHIKV-pseudotyped lentiviral vector at various concentrations were mixed with 60 μL IC RDT extraction buffer in a tube. The IC dipstick then was inserted into the tube of diluted supernatant to start the reaction. After 15 min, the appearance of the control and test lines was assessed. An IC Reader also was used to quantitate the intensities of the test lines; values were expressed as milli-absorbance units (mAbs).

Copyright and License information: The Author(s) ©2020
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