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Evans blue dye was used to assess BBB permeability in mice in vivo. Directly after stereotactic injection, Evans blue dye (EBD) (2%; 4 mL/kg) was injected into the tail vein. At the end of the study, the mice in all three groups were sacrificed, and the tissues were perfused with saline. The brains of the mice were quickly harvested and then weighed and homogenized in 50% trichloroacetic acid (TCA). The concentration of EBD was indexed by measuring the fluorescence intensity of the dye with excitation at 620 nm and emission at 680 nm.
Copyright and License information: ©2020 Liu et al.
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