LinRegPCR analysis of RT-qPCR data

Step One plus experimental files were exported and reformatted for LinRegPCR analysis and determination of Cq (Tuomi et al., 2010). Baselines were determined and amplicon groups were assigned with the following exclusion criteria; samples under analysis; samples without amplification, samples without plateau phase, samples with low Cq value and samples being outside of the 5% of the group median efficiency per amplicon. Furthermore, a log linear phase parameter during estimation of baseline was included. The qPCR efficiencies were exported and statistically analysed.