We treated HepG2 cells with 2, 5, 10, and 20 µM lapatinib for 2, 6, or 24 h. The stock solutions (1,000X) were prepared in dimethyl sulfoxide (DMSO) and stored at −20°C. Accordingly, we used 0.1% (v/v) DMSO as a negative control treatment.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.