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SY Sunil Kumar Yadav
JD Joyati Das
RK Rahul Kumar
GJ Gopaljee Jha
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The bacterium Burkholderia gladioli strain NGJ1 and its derivative strains were grown on PDA (Potato Dextrose Agar; Himedia, India) or CDA (Czapek dox agar; Sucrose: 3%, NaNO3: 0.2%, K2PO4: 0.1%, MgSO4: 0.05%, KCl: 0.05%, FeSO4: 0.001% and Agar: 1.5%; Himedia, India) plates at 28 °C. Whenever required, the media was supplemented with different antibiotics (Kanamycin: 50 μg/ml; Rifampicin: 50 μg/ml and Spectinomycin: 50 μg/ml). The fungus Rhizoctonia solani strain BRS1 was grown on PDA or CDA plates at 28 °C. Generally R. solani produces plenty of sclerotia, the spore-like resting structures on PDA/CDA plates. Germination of sclerotia produces mycelia which form secondary sclerotia at maturity. The bacterial strains and plasmids used in this study are listed in Additional file: Table S7.

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