Antibodies used for Western blot analyses were purchased from the following companies: Akt, pS473-Akt, Smad3, pSmad3, YAP1 and p(S127)YAP1 (Cell Signaling, Beverly, MA); CTGF (Abcam, Cambridge, MA); horseradish peroxidase-labeled secondary antibodies (Promega, Madison, WI). Primary antibodies were used at a 1:1000 dilution for immunoblotting.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.