Human endothelial cells were cultured on coverslips in 24-well plates. Upon reaching confluency, the cells were treated with Peptides 1–5 and ferryl hemoglobin for 6 h. Human blood-derived monocytes were collected from the blood of healthy donors. Phase centrifugation with Histopaque-1077 was used to separate monocytes. Mononuclear cells were suspended in serum-free Dulbecco’s Modified Eagle Medium. Then, mononuclear cells were incubated with calcein-AM for 30 min at 37 °C. Labeled monocytes (2 × 105 cells/well) were added to human endothelial cells in complete culture medium and incubated for 30 min at 37 °C. After that, the cells were fixed with 3.7% formaldehyde.
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