Monocyte adhesion assay

NP Niké Posta
ÉC Éva Csősz
MO Melinda Oros
DP Dávid Pethő
LP László Potor
GK Gergő Kalló
ZH Zoltán Hendrik
KS Katalin Éva Sikura
GM Gábor Méhes
CT Csaba Tóth
JP József Posta
GB György Balla
JB József Balla
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Human endothelial cells were cultured on coverslips in 24-well plates. Upon reaching confluency, the cells were treated with Peptides 1–5 and ferryl hemoglobin for 6 h. Human blood-derived monocytes were collected from the blood of healthy donors. Phase centrifugation with Histopaque-1077 was used to separate monocytes. Mononuclear cells were suspended in serum-free Dulbecco’s Modified Eagle Medium. Then, mononuclear cells were incubated with calcein-AM for 30 min at 37 °C. Labeled monocytes (2 × 105 cells/well) were added to human endothelial cells in complete culture medium and incubated for 30 min at 37 °C. After that, the cells were fixed with 3.7% formaldehyde.

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