For tissue analysis, samples (healthy arteries (n = 6), atheromatous plaques (n = 5), and hemorrhaged complicated lesions (n = 10)) were homogenized in liquid nitrogen. For cerebrospinal fluid analysis, healthy (n = 7), early-phase IVH (n = 6), and late-phase IVH (n = 6) samples were collected. After electrophoresis, the gel was fixed in fixing solution (50% methanol and 10% glacial acetic acid) for 1 h and incubated in staining solution (0.1% Coomassie Brilliant Blue R-250, 50% methanol and 10% glacial acetic acid) for 45 min with shaking. The gel was destained with destaining solution (40% methanol and 10% glacial acetic acid) until no background staining was visible to visualize protein bands.
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