Coomassie brilliant blue staining

NP Niké Posta
ÉC Éva Csősz
MO Melinda Oros
DP Dávid Pethő
LP László Potor
GK Gergő Kalló
ZH Zoltán Hendrik
KS Katalin Éva Sikura
GM Gábor Méhes
CT Csaba Tóth
JP József Posta
GB György Balla
JB József Balla
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For tissue analysis, samples (healthy arteries (n = 6), atheromatous plaques (n = 5), and hemorrhaged complicated lesions (n = 10)) were homogenized in liquid nitrogen. For cerebrospinal fluid analysis, healthy (n = 7), early-phase IVH (n = 6), and late-phase IVH (n = 6) samples were collected. After electrophoresis, the gel was fixed in fixing solution (50% methanol and 10% glacial acetic acid) for 1 h and incubated in staining solution (0.1% Coomassie Brilliant Blue R-250, 50% methanol and 10% glacial acetic acid) for 45 min with shaking. The gel was destained with destaining solution (40% methanol and 10% glacial acetic acid) until no background staining was visible to visualize protein bands.

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