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HeLa and U-2OS cells were grown in Dulbecco’s modified Eagle medium (DMEM; Gibco, catalog no. 21063029), supplemented with 10% fetal bovine serum (FBS; Gibco, catalog no. 10438026), and 1% mL/L penicillin-streptomycin (Gibco, catalog no. 15140122) at 37 °C with 5% CO2. Cells were passaged twice to three times a week and used between passage number 2 and 20. Passaging was performed using 1× PBS and 0.05% Trypsin-EDTA (Gibco, catalog no. 25300054). Approximately 24 h before fixation, cells were seeded on fibronectin-coated glass coverslips at ~65,000 cells per well.
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