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Human adhesion fibroblast cytokine analysis
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Freshly isolated human adhesion fibroblasts were plated in antibiotic-free media and cell supernatant was collected at 24 and 48 h after plating, as well as cell-free media negative control. The 62-plex Luminex assay (custom-built by eBioscience) to assess cytokine content in the supernatant was conducted per the manufacturer’s protocol. Three biological replicates were analyzed per timepoint. Median fluorescence intensity was used for quantification.
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