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Tissue processing and histology
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Mouse and human adhesion and control tissues were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) for 20 h at 4 °C and embedded into paraffin per standard protocols. For cryopreservation, specimens were placed in 30% sucrose (Sigma) until saturation at 4 °C following fixation, followed by OCT until saturation at 4 °C, and then embedded in OCT. Representative tissue specimens were stained with hematoxylin and eosin (H&E, Sigma–Aldrich), Picrosirius Red Stain (Abcam), or Masson’s trichrome (Sigma–Aldrich) per manufacturer’s protocols.
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