4.5. Cell Proliferation Assay with CFSE Staining

The cells were cultured in six-well plates in RPMI 1640 medium with 5% (v/v) FBS at a density of 50,000 cells per well and stained with CFSE for 48 h. Cells were then incubated with 0.1% DMSO or compound treatment for another 24 or 48 h. After the treatment, cell proliferation was detected by FACSan FL1 channel (Becton Dickinson, San Jose, CA, USA). Proliferation index was analyzed with software ModFit LT^TM 3.3 (Verity Software House, Topsham, ME, USA) to quantify the inhibition of cell proliferation.