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Wound-healing scratch assay

SA Shogo Amano
SK Seiji Kaino
SS Shuhei Shinoda
HH Hirofumi Harima
TM Toshihiko Matsumoto
KF Koichi Fujisawa
TT Taro Takami
NY Naoki Yamamoto
TY Takahiro Yamasaki
IS Isao Sakaida
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The migratory ability of pancreatic cancer cells was evaluated using a wound-healing assay. Each pancreatic cancer cell line was cultured at 37 °C and 5% CO2 in 6-well culture plates (BD Biosciences, San Jose, CA, USA) to 80% confluence. The suspended cells were removed with three washes of phosphate-buffered saline (PBS). A sterilised pipette tip was then used to create a wound (scratch) in the confluent layer. Next, DFX (0, 10, 50, or 100 μM), the Rac-1 inhibitor NSC 23766 (0, 50, 100, 200 μM; Selleck, Houston, TX, USA), or the Cdc42 inhibitor ML141 (0, 10, 20, 40 μM; Selleck) were added to each well, and the cells were incubated for 24 h. Afterwards, the wound width was measured; the ratio of pre- and post-culture wound widths was used as an index of cell migration, and comparisons were made with the control group.

Copyright and License information: The Author(s) ©2020
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