IOP Measurement

Mice were put into the Decapicone, a plastic bag especially for the mouse, which could fully expose the head but restrict its movement (Wang et al., 2005). The head of conscious mice was exposed in the hole at the top of the cone and IOP was measured as soon as the mice stayed stable. An effective reading of daytime IOP was obtained weekly at 10 am and 2 pm by applying a probe of tonometer (TonoLab, Colonial Medical, USA) to gently flatten an area of the corneal surface. After baseline IOP was obtained, the right eye was then treated with DEX-Ace or vehicle suspension every 4 d. The IOP of each eye was taken from the average of 3 test values.