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Animal model and iMSC-sEV administration

YX Yuguo Xia
XL Xiaozheng Ling
GH Guowen Hu
QZ Qingwei Zhu
JZ Juntao Zhang
QL Qing Li
BZ Bizeng Zhao
YW Yang Wang
ZD Zhifeng Deng
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All animal experiments were approved by the Animal Research Committee of the Shanghai Sixth People’s Hospital (SYXK [Shanghai, China] 2011-0128, 1 January 2011). Male Sprague Dawley (SD) rats (6–8 weeks old, 250–300 g) were randomly assigned to sham or transient middle cerebral artery occlusion (MCAO) groups with different treatments (vehicle or iMSC-sEV tail veil injection) by using a lottery drawing box. Transient focal cerebral ischemia was induced by 2 h MCAO as previously described [34]. Rats in the sham group underwent the same procedure without vascular occlusion. Rectal temperature was monitored and maintained at 37.0 ± 0.5 °C during the entire procedure using a temperature-controlled heating pad. Rats showing no neurological deficits or dead post-MCAO were excluded for data analysis. iMSC-sEV (1 × 1011 particles in 500 μL PBS) or vehicle (PBS alone, 500 μL) were administered via tail vein injection 4 h after MCAO based on our previous experience [35].

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