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Log-phase bacteria (5 × 105 CFU/ml) were incubated with 6.25 μM of HPN-4fl, HNP-41–11, HNP-41–11mod or 0.01% acetic acid as a control in 10 mM sodium phosphate buffer containing 1% (w/v) TSB. After incubation at 37°C and 150 rpm for 0 to 120 min, a sample was taken from the suspension and added to a 0.05% (v/v) sodium polyanethole sulfonate (Sigma-Aldrich) solution, which neutralizes remaining peptide activity, and plated on LB agar to determine the number of viable bacteria. Experiments were carried out at least three independent times.
Copyright and License information: ©2020 Ehmann, Koeninger, Wendler, Malek, Stange, Wehkamp and Jensen.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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