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ELISA for Quantitative Analysis of IL-6, IL-10, and TNF-α
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The contents of interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α in blood serum of mice and culture medium of RAW264.7 cells were measured by Enzyme-linked immunosorbent assay (ELISA) kits (Beyotime, China) according to the manufacturer’s instructions.
Mouse blood serum was obtained as described before, and culture medium of cells was derived as follows: RAW264.7 cells (4×105 cells/well) in 96-well plates were treated with various concentrations of THD for 0.5 h, followed by incubation with 100 µg/ml LPS for 23.5 h.
Copyright and License information: ©2020 Wang, Jia, Zhang, Wei and Liu
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