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Microscopic images were acquired using a Nikon stereomicroscope (Mellville, NY, USA). For the one-cell stage (WT n = 10, appb26_2/26_2 n = 8), the size of the embryo was measured across the yolk. The total body length of 24 hpf (WT n = 20, appb26_2/26_2 n = 20), 48 hpf (WT n = 15, appb26_2/26_2 n = 19) and 3 dpf (WT n = 15, appb26_2/26_2 n = 19) embryos was measured from the apical part of the head to the caudal end of the tail fin, along the anterior-posterior axis. The 24 hpf was repeated once and other time points performed once.
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