2.8. COX1 and COX2 Enzymatic Assay

COX1 and COX2 inhibition assay used the COX Activity Assay kit (cat # 760151, Cayman Chemicals, Ann Arbor, MI, USA) to investigate the OC treatments effect on the enzymatic activity of COX enzymes versus the standard COX inhibitors DuP-697 (Cayman Chemicals, cat # 70645) and SC-560 (Cayman Chemicals, cat # 70340). DuP-697 is a selective COX2 diaryl heterocyclic inhibitor while the SC-560 is a selective COX-1 inhibitor. Cells lysate samples were processed and each sample was assessed run as described by Cayman Chemicals. The colorimetric COX assay was measured by monitoring the appearance of colorimetric oxidized N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) at 590 nm, which was translated to COX activity as per the manufacturer’s protocol.