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Preparation of palmitic acid

HK Hae-Suk Kim
GR Guang Ren
TK Teayoun Kim
SB Sushant Bhatnagar
QY Qinglin Yang
YB Young Yil Bahk
JK Jeong-a Kim
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Preparation of PA was carried out as described by Mott et al.81. Briefly, 10.5% BSA (Fitzgerald, MA, USA) was dissolved in 25 mM HEPES/DMEM, and filtered (0.22 μM, Millipore, Burlington, MA, USA). Sodium palmitate was heated to be dissolved in water and rapidly added to warmed BSA solution. Then this BSA-conjugated PA, oleic acid, or lauric acid (Nu-Chek, Elysian, MA, USA) were added to the concentrations as indicated in the figure legends. We used endotoxin-free reagents and we tested all the reagents we used including BSA, PA, media and reagent diluents. We checked the endotoxin level of all the reagents we used in this study by Chromogenic Endotoxin Quantitation assay kit (Thermo Fisher, Waltham, MA, USA). Lower than 25 pg/ml of endotoxin is undetectable.

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