Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Preparation of Aβ(1–42) amyloid fibril seed

KI Koki Ikeda
SS Shota Suzuki
YS Yoshiki Shigemitsu
TT Takeshi Tenno
NG Natsuko Goda
AO Atsunori Oshima
HH Hidekazu Hiroaki
request Request a Protocol
ask Ask a question
Favorite

The amyloid fibril seed for the seeding experiment was prepared from the human Aβ(1–42) peptide according to the self-template protocol over the fourth generation35,36. The first-ancestor fibrils were grown by incubation of fresh monomer Aβ(1–42) solutions at 37 ℃ for periods of 48 h in an unstirred and unshaken tube The second generation fibrils were grown under the same condition by seeding fresh solutions with sonicated fragments (5% of total Aβ(1–42)) of the first generation fibrils. This process was repeated three times, and the fourth-generation fibrils were fragmented by sonication to prepare the fibrillization seeds.

Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A