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5.6. Measurement of Reactive Oxygen Species (ROS) Level
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Fresh kidney tissues were mounted using Tissue-Tek O.C.T. Unfixed frozen samples were cut into 5 μm thick sections and placed on glass slides. DHE (dihydroethidium, 10 μmol/L) was applied to each tissue section, and then, the slides were incubated in a light-protected humidified chamber at 37°C for 30 min. The resulting color reaction was immediately measured with a fluorescence microscopy (Leica, Germany) and analyzed by image densitometry using ImageJ software (NIH).
Copyright and License information: ©2020 Zhang Yifan et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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