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HCT116 and SW620 cells were collected following transfection for 48 hrs, and fixed using ice‐cold ethanol (70%) for 24 h at −20°C. Next, the cells were collected and washed by PBS, and then stained with 25 μg/mL propidium iodide (PI) solution in PBS containing 0.2% Triton X‐100 and 50 μg/mL RNase for 20 min in the darkness. Lastly, flow cytometry (Guava Technologies, Hayward, CA, USA) was used to analyze the cell cycle distribution.
Copyright and License information: ©2020 Wang et al.
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