Plant materials

Wheat differential cultivars ‘Nambukomugi’, ‘Fukuhokomugi’, and ‘Hokkai 240’ were used to identify the WYMV pathotype of our field nurseries. For genetic analysis, ‘OW104’, a WYMV-resistant wheat cultivar, and ‘Hokushin’, a susceptible cultivar, were used. A population of 221 F₃ progenies was developed from a cross between ‘OW104’ and ‘Hokushin’. ‘Hokushin’ NILs were developed by backcrossing, with ‘OW104’ as the resistance donor and ‘Hokushin’ as the recurrent parent. BC_nF₁ NILs were evaluated for WYMV resistance in field experiments, and only resistant plants were backcrossed further.