Determination of IL-1β, IL-6, TNF-α and cAMP levels in the hippocampus

Cytokine and cAMP concentrations in the mouse hippocampus were measured by ELISA, according to the manufacturers instructions. The following kits were used: Mouse IL-1β ELISA kit (cat. no. 1210122); mouse IL-6 ELISA kit (cat. no. 1210602); mouse TNF-α ELISA kit (cat. no. 1217202; all purchased from Dakewei Biotechnology Co., Ltd.); and mouse cyclic adenosine monophosphate, cAMP GENLISA™ ELISA kit (cat. no. KLM0071; Krishgen BioSystems). The concentrations were measured using a microplate reader (450 nm absorbance). The results were reported in picograms per milliliter (pg/ml).